For HE staining, after drying the slides for thirty seconds at space temperature, they had been positioned in xylene for 1 minute, followed by 100%, 95%, eighty% and 70% ethanol for 1 minute, respectively. Right after dipping in distilled drinking water for 30 seconds, haematoxylin staining was performed for five minutes, dipping into dH2O once more, and then positioning the slides in Eosin resol
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Posted by NielsenMark2 (#3378) 1 hour 25 minutes ago (https://sites.google.com)
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Importance was established employing the unpaired student's t-check. Scale bar suggests five mm. (C,D) T cells from C57Bl/six or C57Bl/six Ly5.one (WT) or B6.PKCh2/two ended up not activated (C) or activated with 300 ng/ml CCL21 for the indicated time details (D). Cells were lysed and analyzed on SDSPAGE, transferred onto PVDF membranes and blotted with anti-actin and anti-phospho-ERM antibodies.
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