The immunohistochemistry for the tissue slides was executed as explained previously by Simmons et al.  employing a rabbit polyclonal anti-MCPH1 antibody (Abcam, Cambridge, MA) at a dilution of 1:a hundred and a secondary antibody from Bangalore GeneiH, Bangalore, India. The degree of MCPH1 staining in tumors was scored as the percentage of cells stained with DAB (3,3-diaminobenzidine) in a wh
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